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Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
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遗传学
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JoVE 杂志 遗传学
Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

16,150 Views

10:50 min

February 25, 2017

DOI:

10:50 min
February 25, 2017

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Summary

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We describe a method to sort single mammalian cells and to quantify the expression of up to 96 target genes of interest in each cell. This method includes the use of internal qPCR standards to enable the estimation of absolute transcript counts.

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