Journal
/
Genética
/
Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
JoVE Revista
Genética
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Genética
Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

16,166 Views

•

10:50 min

•

February 25, 2017

DOI:

10.3791/55219-v

DOI:
10.3791/55219-v

•

10:50 min
February 25, 2017

•

9 Views
, ,
1Laboratory of Pathology, Center for Cancer Research,National Cancer Institute, 2Experimental Transplantation and Immunology Branch, Center for Cancer Research,National Cancer Institute

Summary

Automatically generated

We describe a method to sort single mammalian cells and to quantify the expression of up to 96 target genes of interest in each cell. This method includes the use of internal qPCR standards to enable the estimation of absolute transcript counts.

Explore More Videos

Single-cell Gene ExpressionFACSQPCRInternal StandardsMicrofluidicTranscript QuantificationRNA-SeqCell SortingAmpliconNeocarzinostatinLysis BufferE. Coli DNAStandards

Read Article

Cite this Article

Porter, J. R., Telford, W. G., Batchelor, E. Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards. J. Vis. Exp. (120), e55219, doi:10.3791/55219 (2017).

Download .ris file

Copy

Share Video

.

Copy