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์ฅ ๋ฐฐ์•„ ์ค‘์กฑ๊ณจ์˜ ๋ฌธํ™” : ์—ฐ๊ณจ ๊ณจํ™”์˜ ์ƒ๋ฆฌ ํ•™์  ๋ชจ๋ธ
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal Developmental Biology
Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification
DOI:

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07:23 min

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December 03, 2016

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Chapters

  • 00:05Title
  • 00:51Dissection Preparation
  • 01:47Murine Embryonic Metatarsal Dissection and Culture
  • 04:57Results: A Tool for Understanding Endochondral Ossification
  • 06:20Conclusion

Summary

Automatic Translation

We present a protocol to dissect and culture embryonic day 15 (E15) murine metatarsal bones. This highly physiological ex vivo model of endochondral ossification provides conditions closer to the in vivo situation than cells in monolayer or 3D culture and is a vital tool for investigating bone growth and development.

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