Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

Dean A. Houston; Katherine A. Staines; Vicky E. MacRae; Colin Farquharson

doi:10.3791/54978

Journal

/

Entwicklungsbiologie

/

Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

JoVE Journal
Entwicklungsbiologie

This content is Free Access.

JoVE Journal Entwicklungsbiologie

Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

10,786 Views

•

07:23 min

•

December 03, 2016

DOI:

10.3791/54978-v

DOI:

10.3791/54978-v

•

07:23 min

December 03, 2016

•

3 Views

Dean A. Houston*¹, Katherine A. Staines*¹, Vicky E. MacRae¹, Colin Farquharson¹

¹Developmental Biology, The Roslin Institute and R(D)SVS,The University of Edinburgh

Summary

We present a protocol to dissect and culture embryonic day 15 (E15) murine metatarsal bones. This highly physiological ex vivo model of endochondral ossification provides conditions closer to the in vivo situation than cells in monolayer or 3D culture and is a vital tool for investigating bone growth and development.

Explore More Videos

Murine Embryonic Metatarsals Endochondral Ossification Ex Vivo Model Physiological Model Skeletal Mineralization Dissection Cell-cell Interactions Cell-matrix Interactions Culture Medium Hind Limb Metatarsal Tweezers Dissecting Microscope

Read Article

Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

Summary

Automatically generated

Explore More Videos