Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

Dean A. Houston; Katherine A. Staines; Vicky E. MacRae; Colin Farquharson

doi:10.3791/54978

Journal

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Biologie du développement

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Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

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Biologie du développement

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Journal JoVE Biologie du développement

Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

10,797 Views

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07:23 min

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December 03, 2016

DOI:

10.3791/54978-v

DOI:

10.3791/54978-v

•

07:23 min

December 03, 2016

•

3 Views

Dean A. Houston*¹, Katherine A. Staines*¹, Vicky E. MacRae¹, Colin Farquharson¹

¹Developmental Biology, The Roslin Institute and R(D)SVS,The University of Edinburgh

Summary

We present a protocol to dissect and culture embryonic day 15 (E15) murine metatarsal bones. This highly physiological ex vivo model of endochondral ossification provides conditions closer to the in vivo situation than cells in monolayer or 3D culture and is a vital tool for investigating bone growth and development.

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Murine Embryonic Metatarsals Endochondral Ossification Ex Vivo Model Physiological Model Skeletal Mineralization Dissection Cell-cell Interactions Cell-matrix Interactions Culture Medium Hind Limb Metatarsal Tweezers Dissecting Microscope

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Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

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