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Journal / Developmental Biology / Culture of Murine Embryonic Metatarsals: A…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Culture of Murine Embryonic Metatarsals
 

Culture of Murine Embryonic Metatarsals: A Physiological Model of Endochondral Ossification

Article DOI: 10.3791/54978-v • 07:23 min • December 3rd, 2016
December 3rd, 2016 •
*1, *1, 1, 1
1Developmental Biology, The Roslin Institute and R(D)SVS, The University of Edinburgh
* These authors contributed equally

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Resumo
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We present a protocol to dissect and culture embryonic day 15 (E15) murine metatarsal bones. This highly physiological ex vivo model of endochondral ossification provides conditions closer to the in vivo situation than cells in monolayer or 3D culture and is a vital tool for investigating bone growth and development.

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Culture Metatarsals Embryonic Mice Ex Vivo Model Endochondral Bone Growth Mineralization Ossification Skeletal Mineralization Physiological Model Dissection Medium Alpha Minimum Essential Media PBS BSA Culture Medium Cell To Cell Interactions Cell To Matrix Interactions 2D Culture 3D Culture Dissection Equipment E15 Wild Type C57 Black 6J Embryos Petri Dish
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