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JoVE Journal
Developmental Biology

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Analysis of Coronary Vessels in Cleared Embryonic Hearts
 

Analysis of Coronary Vessels in Cleared Embryonic Hearts

Article DOI: 10.3791/54800-v 08:25 min December 7th, 2016
December 7th, 2016

챕터

요약

We present a protocol for the analysis of coronary vessels in whole embryonic murine hearts up to E15.5, using standard immunological staining methods followed by optical clearance and confocal microscopy. This technique enables visualization of blood vessels throughout the entire heart without the need for time-consuming analysis of serial sections.

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Keywords: Coronary Vessels Tissue Clearing BABB Cardiovascular Development Coronary Heart Disease Embryonic Hearts Immunostaining Whole Mount Primary Antibody
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