Journal
/
발생학
/
Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

14,010 Views

•

10:00 min

•

January 12, 2015

DOI:

10.3791/52087-v

DOI:
10.3791/52087-v

•

10:00 min
January 12, 2015

•

3 Views
, ,
1Max Delbrück Center for Molecular Medicine, 2Institute of Biochemistry and Biology,University of Potsdam, 3Institute of Molecular Biology,Medizinische Hochschule Hannover

Summary

Automatically generated

To analyse cardiac gene expression profiles during zebrafish heart development, total RNA has to be extracted from isolated hearts. Here, we present a protocol for collecting functional/beating hearts by rapid manual dissection from zebrafish embryos to obtain cardiac-specific mRNA.

Explore More Videos

ZebrafishEmbryonic HeartDissectionTranscriptional AnalysisCardiac TranscriptomeRNA ExtractionQuantitative Real-time PCRFluorescent LabelingFluidic Shear ForceFiltration

Read Article

Cite this Article

Lombardo, V. A., Otten, C., Abdelilah-Seyfried, S. Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis. J. Vis. Exp. (95), e52087, doi:10.3791/52087 (2015).

Download .ris file

Copy

Share Video

.

Copy