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Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
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Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

DOI:
10.3791/52087-v

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10:00 min

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January 12, 2015

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, ,
1Max Delbrück Center for Molecular Medicine, 2Institute of Biochemistry and Biology,University of Potsdam, 3Institute of Molecular Biology,Medizinische Hochschule Hannover

Chapters

  • 00:05Title
  • 01:27Dissecting Zebrafish Embryonic Hearts
  • 04:25Isolating mRNA from Dissected Hearts
  • 07:44Results: Transcriptional Analysis of Dissected Embryonic Zebrafish Hearts
  • 09:38Conclusion

Summary

자동 번역

자동 번역

To analyse cardiac gene expression profiles during zebrafish heart development, total RNA has to be extracted from isolated hearts. Here, we present a protocol for collecting functional/beating hearts by rapid manual dissection from zebrafish embryos to obtain cardiac-specific mRNA.

Tags

ZebrafishEmbryonic HeartDissectionTranscriptional AnalysisCardiac TranscriptomeRNA ExtractionQuantitative Real-time PCRFluorescent LabelingFluidic Shear ForceFiltration

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