Journal
/
발생학
/
Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

DOI:
10.3791/55652-v

•

06:05 min

•

May 25, 2017

•

,
1Brain Korea 21 PLUS Project for Medical Science,Yonsei University College of Medicine, 2Department of Anatomy, Brain Research Institute,Yonsei University College of Medicine

Chapters

  • 00:05Title
  • 00:38Human Plasma Acetylated Low Density Lipoprotein Dil Complex (Dil-AcLDL) Injection Preparation
  • 02:27Dil-AcLDL Injection and Xenopus tropicalis Embryo Fixation
  • 03:44Results: Representative Xenopus tropicalis Imaging
  • 05:02Conclusion

Summary

자동 번역

자동 번역

This protocol demonstrates a fluorescence-based method to visualize the vasculature and to quantify its complexity in Xenopus tropicalis. Blood vessels can be imaged minutes after the injection of a fluorescent dye into the beating heart of an embryo after genetic and/or pharmacological manipulations to study cardiovascular development in vivo.

Tags

Artificial IntelligenceAI Writing AssistantsContent GenerationCopywritingTime-savingEfficiencyCreativityWriter’s BlockContent WritingDigital AgenciesBlog PostsSocial Media PostsArticles

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/55652?article_type=v

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development
check_url/kr/55652?article_type=v

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart
check_url/kr/55652?article_type=v

Visualization of Chondrocyte Intercalation and Directional Proliferation via Zebrabow Clonal Cell Analysis in the Embryonic Meckel’s Cartilage
check_url/kr/55652?article_type=v

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
check_url/kr/55652?article_type=v

Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo
check_url/kr/55652?article_type=v

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
check_url/kr/55652?article_type=v

Technique to Target Microinjection to the Developing Xenopus Kidney
check_url/kr/55652?article_type=v

Analysis of Coronary Vessels in Cleared Embryonic Hearts
check_url/kr/55652?article_type=v

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
check_url/kr/55652?article_type=v

Quantitative Whole-mount Immunofluorescence Analysis of Cardiac Progenitor Populations in Mouse Embryos

Read Article