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Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis
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Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis

8,267 Views

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06:05 min

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May 25, 2017

DOI:

10.3791/55652-v

DOI:
10.3791/55652-v

•

06:05 min
May 25, 2017

•

8 Views
,
1Brain Korea 21 PLUS Project for Medical Science,Yonsei University College of Medicine, 2Department of Anatomy, Brain Research Institute,Yonsei University College of Medicine

Summary

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This protocol demonstrates a fluorescence-based method to visualize the vasculature and to quantify its complexity in Xenopus tropicalis. Blood vessels can be imaged minutes after the injection of a fluorescent dye into the beating heart of an embryo after genetic and/or pharmacological manipulations to study cardiovascular development in vivo.

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Cite this Article

Ohk, J., Jung, H. Visualization and Quantitative Analysis of Embryonic Angiogenesis in Xenopus tropicalis. J. Vis. Exp. (123), e55652, doi:10.3791/55652 (2017).

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