/
/
Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy
10,745 Views
•
12:06 min
•
February 01, 2017
DOI:
Summary
Automatically generated
A method to measure protein diffusion in membranes of primary immune cells using fluorescence correlation spectroscopy (FCS) is described. In this paper, the use of antibodies for fluorescent labeling is illustrated.
Explore More Videos
Molecular DiffusionPlasma MembranesPrimary LymphocytesFluorescence Correlation SpectroscopyImmunologyCell BiologyNatural Killer CellsMembrane Receptor DynamicsImmunotherapyCancerPrimary CellsCell LinesConfocal Laser Scanning MicroscopeFCS MeasurementsFluorophoresDyePoly-L-lysineCoverglass SlideObjective Lens
Read Article
Cite this Article
Staaf, E., Bagawath-Singh, S., Johansson, S. Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy. J. Vis. Exp. (120), e54756, doi:10.3791/54756 (2017).
Copy