ジャーナル
/
免疫学と感染
/
Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy

DOI:
10.3791/54756-v

•

12:06 min

•

February 01, 2017

•

, ,
1Department of Microbiology, Tumor and Cell Biology,Karolinska Institutet

章

  • 00:05標題
  • 01:07Starting the FCS System
  • 02:26Pinhole Adjustment
  • 04:41Measure the Transit Time of the Free Fluorophore
  • 05:50Measure the Transit Time of Free Antibody
  • 06:51Cell Measurements
  • 09:47Results: Assessing the Autocorrelation Curve
  • 11:04Conclusion

概要

自動翻訳

自動翻訳

A method to measure protein diffusion in membranes of primary immune cells using fluorescence correlation spectroscopy (FCS) is described. In this paper, the use of antibodies for fluorescent labeling is illustrated.

タグ

Molecular DiffusionPlasma MembranesPrimary LymphocytesFluorescence Correlation Spectroscopy免疫学Cell BiologyNatural Killer CellsMembrane Receptor DynamicsImmunotherapyCancerPrimary CellsCell LinesConfocal Laser Scanning MicroscopeFCS MeasurementsFluorophoresDyePoly-L-lysineCoverglass SlideObjective Lens

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Methods to Assess Beta Cell Death Mediated by Cytotoxic T Lymphocytes

piggyBac Transposon System Modification of Primary Human T Cells

Rapid Analysis of Chromosome Aberrations in Mouse B Lymphocytes by PNA-FISH

Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization

Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data

Phagosome Migration and Velocity Measured in Live Primary Human Macrophages Infected with HIV-1

Visualization of IL-22-expressing Lymphocytes Using Reporter Mice

Small RNA Transfection in Primary Human Th17 Cells by Next Generation Electroporation

Isolating Lymphocytes from the Mouse Small Intestinal Immune System

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Read Article