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Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy
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Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy
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February 01, 2017
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A method to measure protein diffusion in membranes of primary immune cells using fluorescence correlation spectroscopy (FCS) is described. In this paper, the use of antibodies for fluorescent labeling is illustrated.
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Molecular DiffusionPlasma MembranesPrimary LymphocytesFluorescence Correlation SpectroscopyImmunologyCell BiologyNatural Killer CellsMembrane Receptor DynamicsImmunotherapyCancerPrimary CellsCell LinesConfocal Laser Scanning MicroscopeFCS MeasurementsFluorophoresDyePoly-L-lysineCoverglass SlideObjective Lens
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Staaf, E., Bagawath-Singh, S., Johansson, S. Molecular Diffusion in Plasma Membranes of Primary Lymphocytes Measured by Fluorescence Correlation Spectroscopy. J. Vis. Exp. (120), e54756, doi:10.3791/54756 (2017).
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