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A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release
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A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release
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November 21, 2016
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Neutrophil extracellular traps (NETs) are networks of DNA, histones and neutrophil proteins. Although a component of the innate immune response, NETs are implicated in autoimmunity and thrombosis. This protocol describes a simple method for canine neutrophil isolation and quantification of NETs using a microplate fluorescence assay.
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Jeffery, U., Gray, R. D., LeVine, D. N. A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release. J. Vis. Exp. (117), e54726, doi:10.3791/54726 (2016).
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