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A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release
JoVE Journal
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JoVE Journal İmmünoloji ve Enfeksiyon
A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release

A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release

7,921 Views

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06:45 min

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November 21, 2016

DOI:

10.3791/54726-v

DOI:
10.3791/54726-v

•

06:45 min
November 21, 2016

•

2 Views
, ,
1Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine,Iowa State Üniversitesi, 2MRC Centre for Inflammation Research,University of Edinburgh, 3Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State Üniversitesi

Özet

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Neutrophil extracellular traps (NETs) are networks of DNA, histones and neutrophil proteins. Although a component of the innate immune response, NETs are implicated in autoimmunity and thrombosis. This protocol describes a simple method for canine neutrophil isolation and quantification of NETs using a microplate fluorescence assay.

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Jeffery, U., Gray, R. D., LeVine, D. N. A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release. J. Vis. Exp. (117), e54726, doi:10.3791/54726 (2016).

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