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A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release
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JoVE 杂志 免疫与感染
A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release

A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release

7,921 Views

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06:45 min

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November 21, 2016

DOI:

10.3791/54726-v

DOI:
10.3791/54726-v

•

06:45 min
November 21, 2016

•

2 Views
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1Department of Veterinary Microbiology and Preventative Medicine, College of Veterinary Medicine,Iowa State University, 2MRC Centre for Inflammation Research,University of Edinburgh, 3Department of Veterinary Clinical Sciences, College of Veterinary Medicine,Iowa State University

Summary

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Neutrophil extracellular traps (NETs) are networks of DNA, histones and neutrophil proteins. Although a component of the innate immune response, NETs are implicated in autoimmunity and thrombosis. This protocol describes a simple method for canine neutrophil isolation and quantification of NETs using a microplate fluorescence assay.

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Jeffery, U., Gray, R. D., LeVine, D. N. A Simple Fluorescence Assay for Quantification of Canine Neutrophil Extracellular Trap Release. J. Vis. Exp. (117), e54726, doi:10.3791/54726 (2016).

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