Journal
/
생화학
/
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
JoVE 신문
생화학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생화학
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

DOI:
10.3791/54527-v

•

08:32 min

•

October 23, 2016

•

, , ,
1Department of Molecular, Cellular, and Developmental Biology,University of Michigan, 2Howard Hughes Medical Institute,University of Michigan

Chapters

  • 00:05Title
  • 01:16Chaperone Activity Assay Using Thermally Unfolding Malate Dehydrogenase (MDH)
  • 03:18Effect of HdeB Overexpression on E. coli Survival under Acid Stress
  • 05:14Results: Chaperone Activity Assay Using Thermally Unfolded MDH and the Effect of HdeB Overexpression on E. coli Survival under Acid Stress
  • 07:16Conclusion

Summary

자동 번역

자동 번역

This study describes biophysical, biochemical and molecular techniques to characterize the chaperone activity of Escherichia coli HdeB under acidic pH conditions. These methods have been successfully applied for other acid-protective chaperones such as HdeA and can be modified to work for other chaperones and stress conditions.

Tags

Keywords: Escherichia ColiChaperone HdeBPH-dependent ActivityIn VitroIn VivoAcid Activated ChaperonesProtein UnfoldingProtein DegradationEnteric BacteriaStomachAcid Protective ChaperonesHDAMalate DehydrogenaseLight ScatteringFluorescent SpectrophotometerTemperature Control

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/54527?article_type=v

Directed Protein Packaging within Outer Membrane Vesicles from Escherichia coli: Design, Production and Purification
check_url/kr/54527?article_type=v

Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli
check_url/kr/54527?article_type=v

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
check_url/kr/54527?article_type=v

Defining Hsp33’s Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
check_url/kr/54527?article_type=v

Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee
check_url/kr/54527?article_type=v

Quantification of Hypopigmentation Activity In Vitro
check_url/kr/54527?article_type=v

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
check_url/kr/54527?article_type=v

Site-Directed Mutagenesis for In Vitro and In Vivo Experiments Exemplified with RNA Interactions in Escherichia Coli
check_url/kr/54527?article_type=v

Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli
check_url/kr/54527?article_type=v

Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor

Read Article