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Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
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Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
Capitoli
- 00:05Titolo
- 01:16Chaperone Activity Assay Using Thermally Unfolding Malate Dehydrogenase (MDH)
- 03:18Effect of HdeB Overexpression on E. coli Survival under Acid Stress
- 05:14Results: Chaperone Activity Assay Using Thermally Unfolded MDH and the Effect of HdeB Overexpression on E. coli Survival under Acid Stress
- 07:16Conclusion
This study describes biophysical, biochemical and molecular techniques to characterize the chaperone activity of Escherichia coli HdeB under acidic pH conditions. These methods have been successfully applied for other acid-protective chaperones such as HdeA and can be modified to work for other chaperones and stress conditions.
