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Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
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Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

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08:32 min

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October 23, 2016

DOI:

10.3791/54527-v

DOI:
10.3791/54527-v

•

08:32 min
October 23, 2016

•

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1Department of Molecular, Cellular, and Developmental Biology,University of Michigan, 2Howard Hughes Medical Institute,University of Michigan

Tags

Escherichia ColiChaperone HdeBPH-dependent ActivityIn VitroIn VivoAcid Activated ChaperonesProtein UnfoldingProtein DegradationEnteric BacteriaStomachAcid Protective ChaperonesHDAMalate DehydrogenaseLight ScatteringFluorescent SpectrophotometerTemperature Control

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Dahl, J., Koldewey, P., Bardwell, J. C. A., Jakob, U. Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo. J. Vis. Exp. (116), e54527, doi:10.3791/54527 (2016).

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