Determinação das espécies e quantificação de misturas utilizando MRM Espectrometria de Massa de peptídeos Aplicada à autenticação Meat

We present a protocol for identifying and quantifying the components in mixtures of species possessing similar proteins. Mass spectrometry detects peptides for identification, and gives relative quantitation by ratios of peak areas. As a tool food for fraud detection, the method can detect 1% horse in beef.