Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

Yvonne Gunning; Andrew D. Watson; Neil M. Rigby; Mark Philo; Joshua K. Peazer; E. Kate Kemsley

doi:10.3791/54420

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Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

JoVE Journal
生化学

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JoVE Journal 生化学

Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

11,252 Views

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09:26 min

•

September 20, 2016

DOI:

10.3791/54420-v

DOI:

10.3791/54420-v

•

09:26 min

September 20, 2016

•

5 Views

Yvonne Gunning¹, Andrew D. Watson¹, Neil M. Rigby², Mark Philo¹, Joshua K. Peazer^1,3, E. Kate Kemsley¹

¹Analytical Sciences Unit,Institute of Food Research, ²Institute of Food Research, ³School of Chemistry,University of East Anglia

概要

We present a protocol for identifying and quantifying the components in mixtures of species possessing similar proteins. Mass spectrometry detects peptides for identification, and gives relative quantitation by ratios of peak areas. As a tool food for fraud detection, the method can detect 1% horse in beef.

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Species Determination and Quantitation in Mixtures Using MRM Mass Spectrometry of Peptides Applied to Meat Authentication

概要

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