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机器人平台的高通量原生质体分离和转型
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A Robotic Platform for High-throughput Protoplast Isolation and Transformation
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机器人平台的高通量原生质体分离和转型

DOI:
10.3791/54300-v

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10:12 min

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September 27, 2016

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, ,
1Department of Plant Sciences,University of Tennessee, Knoxville, 2Center for Renewable Carbon,University of Tennessee, Knoxville, 3Department of Mechanical, Aerospace, and Biomedical Engineering,University of Tennessee, Knoxville

Chapters

  • 00:05Title
  • 00:40Establishment of Suspension Cell Cultures
  • 01:40Set up of Automated Protocols for Protoplast Isolation and Transformation
  • 05:16Automated Protocols
  • 06:37Microscopic Analysis of Transformation
  • 07:42Results: Rapid, Automated, High-throughput Screening of Plant Protoplasts
  • 09:40Conclusion

Summary

자동 번역

자동 번역

高通量，自动化，烟草原生质体的生产和改造的方法进行描述。该机器人系统可以在模型BY-2系统应该是翻译到非模式作物大规模并行基因表达和发现。

Tags

Protoplast IsolationHigh-throughput ScreeningPlant BiotechnologyAutomated Protoplast ProductionProtoplast TransformationBY-2 Suspension CultureMicroplate MoverLiquid HandlerMulti-mode Plate ReaderMulti-mode DispenserPlate HeaterPlate ChillerRobotics SystemPlate Mover SoftwareLabwareContainer TypesStart And End Positions

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