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A Robotic Platform for High-throughput Protoplast Isolation and Transformation
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JoVE Revista Genética
A Robotic Platform for High-throughput Protoplast Isolation and Transformation

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

13,946 Views

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10:12 min

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September 27, 2016

DOI:

10.3791/54300-v

DOI:
10.3791/54300-v

•

10:12 min
September 27, 2016

•

3 Views
, ,
1Department of Plant Sciences,University of Tennessee, Knoxville, 2Center for Renewable Carbon,University of Tennessee, Knoxville, 3Department of Mechanical, Aerospace, and Biomedical Engineering,University of Tennessee, Knoxville

Summary

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A high-throughput, automated, tobacco protoplast production and transformation methodology is described. The robotic system enables massively parallel gene expression and discovery in the model BY-2 system that should be translatable to non-model crops.

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Dlugosz, E. M., Lenaghan, S. C., Stewart, Jr., C. N. A Robotic Platform for High-throughput Protoplast Isolation and Transformation. J. Vis. Exp. (115), e54300, doi:10.3791/54300 (2016).

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