Journal
/
유전학
/
A Robotic Platform for High-throughput Protoplast Isolation and Transformation
JoVE 신문
유전학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 유전학
A Robotic Platform for High-throughput Protoplast Isolation and Transformation

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

13,946 Views

•

10:12 min

•

September 27, 2016

DOI:

10.3791/54300-v

DOI:
10.3791/54300-v

•

10:12 min
September 27, 2016

•

3 Views
, ,
1Department of Plant Sciences,University of Tennessee, Knoxville, 2Center for Renewable Carbon,University of Tennessee, Knoxville, 3Department of Mechanical, Aerospace, and Biomedical Engineering,University of Tennessee, Knoxville

Summary

Automatically generated

A high-throughput, automated, tobacco protoplast production and transformation methodology is described. The robotic system enables massively parallel gene expression and discovery in the model BY-2 system that should be translatable to non-model crops.

Explore More Videos

Protoplast IsolationHigh-throughput ScreeningPlant BiotechnologyAutomated Protoplast ProductionProtoplast TransformationBY-2 Suspension CultureMicroplate MoverLiquid HandlerMulti-mode Plate ReaderMulti-mode DispenserPlate HeaterPlate ChillerRobotics SystemPlate Mover SoftwareLabwareContainer TypesStart And End Positions

Read Article

Cite this Article

Dlugosz, E. M., Lenaghan, S. C., Stewart, Jr., C. N. A Robotic Platform for High-throughput Protoplast Isolation and Transformation. J. Vis. Exp. (115), e54300, doi:10.3791/54300 (2016).

Download .ris file

Copy

Share Video

.

Copy