Journal
/
Génétique
/
A Robotic Platform for High-throughput Protoplast Isolation and Transformation
Journal JoVE
Génétique
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Génétique
A Robotic Platform for High-throughput Protoplast Isolation and Transformation

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

DOI:
10.3791/54300-v

•

10:12 min

•

September 27, 2016

•

, ,
1Department of Plant Sciences,University of Tennessee, Knoxville, 2Center for Renewable Carbon,University of Tennessee, Knoxville, 3Department of Mechanical, Aerospace, and Biomedical Engineering,University of Tennessee, Knoxville

Chapitres

  • 00:05Titre
  • 00:40Establishment of Suspension Cell Cultures
  • 01:40Set up of Automated Protocols for Protoplast Isolation and Transformation
  • 05:16Automated Protocols
  • 06:37Microscopic Analysis of Transformation
  • 07:42Results: Rapid, Automated, High-throughput Screening of Plant Protoplasts
  • 09:40Conclusion

Summary

Traduction automatique

Traduction automatique

A high-throughput, automated, tobacco protoplast production and transformation methodology is described. The robotic system enables massively parallel gene expression and discovery in the model BY-2 system that should be translatable to non-model crops.

Tags

Protoplast IsolationHigh-throughput ScreeningPlant BiotechnologyAutomated Protoplast ProductionProtoplast TransformationBY-2 Suspension CultureMicroplate MoverLiquid HandlerMulti-mode Plate ReaderMulti-mode DispenserPlate HeaterPlate ChillerRobotics SystemPlate Mover SoftwareLabwareContainer TypesStart And End Positions

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Perturbations of Circulating miRNAs in Irritable Bowel Syndrome Detected Using a Multiplexed High-throughput Gene Expression Platform

High-Throughput Robotically Assisted Isolation of Temperature-sensitive Lethal Mutants in Chlamydomonas reinhardtii

High Fat Diet Feeding and High Throughput Triacylglyceride Assay in Drosophila Melanogaster

Generation of Native Chromatin Immunoprecipitation Sequencing Libraries for Nucleosome Density Analysis

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

High-throughput Identification of Synergistic Drug Combinations by the Overlap2 Method

The Replica Set Method: A High-throughput Approach to Quantitatively Measure Caenorhabditis elegans Lifespan

High-throughput Identification of Gene Regulatory Sequences Using Next-generation Sequencing of Circular Chromosome Conformation Capture (4C-seq)

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology

Read Article