Journal
/
유전학
/
In Vitro Транскрипция Анализы и их применение в Drug Discovery
JoVE 신문
유전학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 유전학
In Vitro Transcription Assays and Their Application in Drug Discovery
Please note that all translations are automatically generated. Click here for the English version.

In Vitro Транскрипция Анализы и их применение в Drug Discovery

14,849 Views

•

09:28 min

•

September 20, 2016

DOI:

10.3791/54256-v

DOI:
10.3791/54256-v

•

09:28 min
September 20, 2016

•

4 Views
,
1School of Environmental and Life Sciences,University of Newcastle, 2Department of Applied Biology and Chemical Technology, The State Key Laboratory of Chirosciences,The Hong Kong Polytechnic University

Summary

Automatically generated

In this manuscript, we describe a protocol to functionally examine transcription and the inhibitory activity of antibacterial agents targeting bacterial transcription.

Explore More Videos

In Vitro Transcription AssayTranscription RegulationTranscription FactorsTranscription InhibitorsMolecular BiologyRNA Sequencing GelRNA PolymeraseSigma 70 FactorOpen ComplexTranscription BufferGTPUTPATPP32-labeled UTP

Read Article

Cite this Article

Yang, X., Ma, C. In Vitro Transcription Assays and Their Application in Drug Discovery. J. Vis. Exp. (115), e54256, doi:10.3791/54256 (2016).

Download .ris file

Copy

Share Video

.

Copy