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In Vitro Transcription Assays and Their Application in Drug Discovery
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JoVE Journal Genetik
In Vitro Transcription Assays and Their Application in Drug Discovery

In Vitro Transcription Assays and Their Application in Drug Discovery

14,878 Views

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09:28 min

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September 20, 2016

DOI:

10.3791/54256-v

DOI:
10.3791/54256-v

•

09:28 min
September 20, 2016

•

4 Views
,
1School of Environmental and Life Sciences,University of Newcastle, 2Department of Applied Biology and Chemical Technology, The State Key Laboratory of Chirosciences,The Hong Kong Polytechnic University

Summary

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In this manuscript, we describe a protocol to functionally examine transcription and the inhibitory activity of antibacterial agents targeting bacterial transcription.

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In Vitro Transcription AssayTranscription RegulationTranscription FactorsTranscription InhibitorsMolekularbiologieRNA Sequencing GelRNA PolymeraseSigma 70 FactorOpen ComplexTranscription BufferGTPUTPATPP32-labeled UTP

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Yang, X., Ma, C. In Vitro Transcription Assays and Their Application in Drug Discovery. J. Vis. Exp. (115), e54256, doi:10.3791/54256 (2016).

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