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In Vitro Transcription Assays and Their Application in Drug Discovery
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遗传学
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JoVE 杂志 遗传学
In Vitro Transcription Assays and Their Application in Drug Discovery

In Vitro Transcription Assays and Their Application in Drug Discovery

DOI:
10.3791/54256-v

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09:28 min

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September 20, 2016

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,
1School of Environmental and Life Sciences,University of Newcastle, 2Department of Applied Biology and Chemical Technology, The State Key Laboratory of Chirosciences,The Hong Kong Polytechnic University

Chapters

  • 00:05Title
  • 00:51Preparation of RNA Sequencing Gel
  • 02:08Transcription Elongation Pausing Assay
  • 04:30RNA Gel Run and Gel Development
  • 06:46Results: NusA Regulates Transcription Pausing
  • 08:34Conclusion

Summary

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In this manuscript, we describe a protocol to functionally examine transcription and the inhibitory activity of antibacterial agents targeting bacterial transcription.

Tags

In Vitro Transcription AssayTranscription RegulationTranscription FactorsTranscription InhibitorsMolecular BiologyRNA Sequencing GelRNA PolymeraseSigma 70 FactorOpen ComplexTranscription BufferGTPUTPATPP32-labeled UTP

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