Journal
/
Genética
/
In Vitro Transcription Assays and Their Application in Drug Discovery
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
In Vitro Transcription Assays and Their Application in Drug Discovery

In Vitro Transcription Assays and Their Application in Drug Discovery

14,878 Views

•

09:28 min

•

September 20, 2016

DOI:

10.3791/54256-v

DOI:
10.3791/54256-v

•

09:28 min
September 20, 2016

•

4 Views
,
1School of Environmental and Life Sciences,University of Newcastle, 2Department of Applied Biology and Chemical Technology, The State Key Laboratory of Chirosciences,The Hong Kong Polytechnic University

Summary

Automatically generated

In this manuscript, we describe a protocol to functionally examine transcription and the inhibitory activity of antibacterial agents targeting bacterial transcription.

Explore More Videos

In Vitro Transcription AssayTranscription RegulationTranscription FactorsTranscription InhibitorsBiologia MolecularRNA Sequencing GelRNA PolymeraseSigma 70 FactorOpen ComplexTranscription BufferGTPUTPATPP32-labeled UTP

Read Article

Cite this Article

Yang, X., Ma, C. In Vitro Transcription Assays and Their Application in Drug Discovery. J. Vis. Exp. (115), e54256, doi:10.3791/54256 (2016).

Download .ris file

Copy

Share Video

.

Copy