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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
Chapters
- 00:05Title
- 00:48Förster Resonance Energy Transfer (FRET) Biosensor Preparation
- 04:25Analysis of Sugar Content of Beverages Using a FRET Biosensor
- 06:31Results: Quantitative Analysis of Sugar Content Using a Protein Biosensor for Portable FRET Analyzer
- 07:49Conclusion
This protocol describes the rapid and highly sensitive quantification of Förster resonance energy transfer (FRET) sensor data using a custom-made portable FRET analyzer. The device was used to detect maltose within a critical temperature range that maximizes detection sensitivity, enabling practical and efficient assessment of sugar content.
