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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
JoVE Journal
Biyokimya
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JoVE Journal Biyokimya
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

DOI:

08:27 min

October 01, 2016

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Bölümler

  • 00:05Başlık
  • 00:48Förster Resonance Energy Transfer (FRET) Biosensor Preparation
  • 04:25Analysis of Sugar Content of Beverages Using a FRET Biosensor
  • 06:31Results: Quantitative Analysis of Sugar Content Using a Protein Biosensor for Portable FRET Analyzer
  • 07:49Conclusion

Özet

Otomatik Çeviri

This protocol describes the rapid and highly sensitive quantification of Förster resonance energy transfer (FRET) sensor data using a custom-made portable FRET analyzer. The device was used to detect maltose within a critical temperature range that maximizes detection sensitivity, enabling practical and efficient assessment of sugar content.

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