This protocol describes the rapid and highly sensitive quantification of Förster resonance energy transfer (FRET) sensor data using a custom-made portable FRET analyzer. The device was used to detect maltose within a critical temperature range that maximizes detection sensitivity, enabling practical and efficient assessment of sugar content.
Kim, H., Han, G. H., Fu, Y., Gam, J., Lee, S. G. Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer. J. Vis. Exp. (116), e54144, doi:10.3791/54144 (2016).