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Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer
JoVE Revista
Bioquímica
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JoVE Revista Bioquímica
Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

Highly Sensitive and Rapid Fluorescence Detection with a Portable FRET Analyzer

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08:27 min

October 01, 2016

DOI:

08:27 min
October 01, 2016

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Summary

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This protocol describes the rapid and highly sensitive quantification of Förster resonance energy transfer (FRET) sensor data using a custom-made portable FRET analyzer. The device was used to detect maltose within a critical temperature range that maximizes detection sensitivity, enabling practical and efficient assessment of sugar content.

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