Journal
/
/
Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders
JoVE 신문
유전학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 유전학
Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders

Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders

12,705 Views

10:07 min

December 05, 2016

DOI:

10:07 min
December 05, 2016

8 Views
,

Summary

Automatically generated

We present simple techniques for isolating giant transcriptionally active lampbrush chromosomes from living oocytes of frogs and salamanders. We describe how to observe these chromosomes "alive" by phase contrast or differential interference contrast, and how to fix them for fluorescent in situ hybridization or immunofluorescent staining.

Read Article