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Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders
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December 05, 2016
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We present simple techniques for isolating giant transcriptionally active lampbrush chromosomes from living oocytes of frogs and salamanders. We describe how to observe these chromosomes "alive" by phase contrast or differential interference contrast, and how to fix them for fluorescent in situ hybridization or immunofluorescent staining.
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Gall, J. G., Nizami, Z. F. Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders. J. Vis. Exp. (118), e54103, doi:10.3791/54103 (2016).
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