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Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders
Journal JoVE
Génétique
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Journal JoVE Génétique
Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders

Isolation of Giant Lampbrush Chromosomes from Living Oocytes of Frogs and Salamanders

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10:07 min

December 05, 2016

DOI:

10:07 min
December 05, 2016

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Summary

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We present simple techniques for isolating giant transcriptionally active lampbrush chromosomes from living oocytes of frogs and salamanders. We describe how to observe these chromosomes "alive" by phase contrast or differential interference contrast, and how to fix them for fluorescent in situ hybridization or immunofluorescent staining.

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