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Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea
Chapters
- 00:05Title
- 00:59Preparation of Temporal Bones
- 02:14Whole Mount Dissection of the Cochlea
- 05:41Immunostaining with Fluorescently Tagged Antibodies
- 07:30Mounting Stained Cochlear Turns
- 08:59Results: Examples of Optimal and Suboptimal Dissection and Immunostaining
- 10:54Conclusion
We present a method to isolate the adult organ of Corti as three intact cochlear turns (apex, middle, and base). We also demonstrate a procedure for immunostaining with fluorescently tagged antibodies. Together these techniques allow the study of hair cells, supporting cells, and other cell types found in the cochlea.
