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Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea

Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea

53,075 Views

12:02 min

January 01, 2016

DOI:

12:02 min
January 01, 2016

56 Views
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Summary

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We present a method to isolate the adult organ of Corti as three intact cochlear turns (apex, middle, and base). We also demonstrate a procedure for immunostaining with fluorescently tagged antibodies. Together these techniques allow the study of hair cells, supporting cells, and other cell types found in the cochlea.

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