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JoVE Journal Neuroscience
Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea
 

Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea

Article DOI: 10.3791/53561-v • 12:02 min • January 1st, 2016
January 1st, 2016 •
1, 1,2
1Department of Surgery, Division of Otolaryngology, Southern Illinois University, School of Medicine, 2Department of Pharmacology, Southern Illinois University, School of Medicine

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Resumen
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We present a method to isolate the adult organ of Corti as three intact cochlear turns (apex, middle, and base). We also demonstrate a procedure for immunostaining with fluorescently tagged antibodies. Together these techniques allow the study of hair cells, supporting cells, and other cell types found in the cochlea.

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Whole Mount Dissection Immunofluorescence Adult Mouse Cochlea Sensory Region Calcified Cochlea Intact Turns Apex Middle Base Organ Of Corti Immunostaining Confocal Microscopy Z Axis Dissection Method Cochlear Turns Visual Demonstration Spiral Ligament Removal Euthanizing Temporal Bones Cranial Nerves Otic Capsule Posterior Semi-circular Canal Encapsulated Cochlea Methanol Free Electron Microscopy Grade 4%paraformaldehyde Fixation
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