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Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals
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Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

17,110 Views

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06:46 min

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January 05, 2016

DOI:

10.3791/53495-v

DOI:
10.3791/53495-v

•

06:46 min
January 05, 2016

•

9 Views
, , , , , ,
1Department of Microbiology, Biochemistry, Immunology,Morehouse School of Medicine, 2Department of Medicine,Emory University School of Medicine, 3Department of Pathology and Laboratory Medicine,Emory University School of Medicine, 4Yerkes National Primate Research Center

Summary

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Techniques describing a gradient procedure to separate exosomes from human immunodeficiency virus (HIV) particles are described. This procedure was used to isolate exosomes away from HIV particles in human plasma from HIV-infected individuals. The isolated exosomes were analyzed for cytokine/chemokine content.

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ExosomesHIV-1PlasmaVirus ParticlesUltracentrifugationIodixanol GradientVirus-infected CellsMicrovesiclesHIV InfectionCentrifugationPBS WashVirus Separation

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Konadu, K. A., Huang, M. B., Roth, W., Armstrong, W., Powell, M., Villinger, F., Bond, V. Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals. J. Vis. Exp. (107), e53495, doi:10.3791/53495 (2016).

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