Dergi
/
İmmünoloji ve Enfeksiyon
/
Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals
JoVE Journal
İmmünoloji ve Enfeksiyon
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal İmmünoloji ve Enfeksiyon
Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

17,110 Views

•

06:46 min

•

January 05, 2016

DOI:

10.3791/53495-v

DOI:
10.3791/53495-v

•

06:46 min
January 05, 2016

•

9 Views
, , , , , ,
1Department of Microbiology, Biochemistry, Immunology,Morehouse School of Medicine, 2Tıp Fakültesi,Emory University School of Medicine, 3Department of Pathology and Laboratory Medicine,Emory University School of Medicine, 4Yerkes National Primate Research Center

Özet

Automatically generated

Techniques describing a gradient procedure to separate exosomes from human immunodeficiency virus (HIV) particles are described. This procedure was used to isolate exosomes away from HIV particles in human plasma from HIV-infected individuals. The isolated exosomes were analyzed for cytokine/chemokine content.

Explore More Videos

ExosomesHIV-1PlasmaVirus ParticlesUltracentrifugationIodixanol GradientVirus-infected CellsMicrovesiclesHIV InfectionCentrifugationPBS WashVirus Separation

Read Article

Cite this Article

Konadu, K. A., Huang, M. B., Roth, W., Armstrong, W., Powell, M., Villinger, F., Bond, V. Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals. J. Vis. Exp. (107), e53495, doi:10.3791/53495 (2016).

Download .ris file

Copy

Share Video

.

Copy