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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals
 

Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals

Article DOI: 10.3791/53495-v • 06:46 min • January 5th, 2016
January 5th, 2016 •
1, 1, 1, 2, 1, 3,4, 1
1Department of Microbiology, Biochemistry, Immunology, Morehouse School of Medicine, 2Department of Medicine, Emory University School of Medicine, 3Department of Pathology and Laboratory Medicine, Emory University School of Medicine, 4Yerkes National Primate Research Center

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Techniques describing a gradient procedure to separate exosomes from human immunodeficiency virus (HIV) particles are described. This procedure was used to isolate exosomes away from HIV particles in human plasma from HIV-infected individuals. The isolated exosomes were analyzed for cytokine/chemokine content.

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Exosomes Plasma HIV-1 Isolation Virus Particles Procedure Plasma Samples HIV Infected Patients Proteins Molecules Dr. Ming Bo Huang Department Of Microbiology Biochemistry And Immunology Moorehouse School Of Medicine Blood Collection Ultracentrifuge Tube Centrifugation Vesicles
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