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Developmental Biology

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JoVE Journal Developmental Biology
Geomagnetic Field (Gmf) and Plant Evolution
 

Geomagnetic Field (Gmf) and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression

Article DOI: 10.3791/53286-v • 11:04 min • November 30th, 2015
November 30th, 2015 •
1, 1, 1, 2, 1
1Department of Life Sciences and Systems Biology, University of Turin, 2Radcliffe Department of Medicine, University of Oxford, John Radcliffe Hospital

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요약
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This protocol describes a method to test the hypothesis that the reversal of the effective geomagnetic field (GMF) induces differential gene expression and alters the morphology of Arabidopsis thaliana. A triaxial octagonal system of Helmholtz coil-pairs was used to artificially generate reversed GMF conditions in the plant growth chamber.

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Geomagnetic Field GMF Reversal Plant Evolution Arabidopsis Thaliana Gene Expression Magnetometer Helmholtz Coil Pairs GMF Conditions Petri Plates Root Length Leaf Areas RNA Extraction QPCR Analyses CRUCIFERIN 3 Copper Transport Protein1 Redox Responsive Transcription Factor1 Fe Superoxide Dismutase 1 Catalase3 Thylakoidal Ascorbate Peroxidase Cytosolic Ascorbate Peroxidase1 NADPH/respiratory Burst Oxidase Protein D
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