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Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System
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Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System

Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System

3,447 Views

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09:00 min

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February 11, 2022

DOI:

10.3791/63509-v

DOI:
10.3791/63509-v

•

09:00 min
February 11, 2022

•

12 Views
, , , ,
1Department of Biology,Reed College, 2Committee on Development, Regeneration, and Stem Cell Biology,University of Chicago, 3Department of Cell and Developmental Biology,University College London

Summary

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The article explains how to surgically remove eyes from living zebrafish larvae as the first step toward investigating how retinal input influences optic tectum growth and development. In addition, the article provides information about larval anesthetization, fixation, and brain dissection, followed by immunohistochemistry and confocal imaging.

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Eye RemovalZebrafish LarvaeInnervation-dependent GrowthVisual SystemRetinal InputOptic TectumNeural DevelopmentRegenerationDegenerationSurgical TechniqueAnesthesiaImmobilizationAgarose

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Cite this Article

Hagen, O. L., Kim, Y., Kushkowski, E., Rouse, H., Cerveny, K. L. Eye Removal in Living Zebrafish Larvae to Examine Innervation-dependent Growth and Development of the Visual System. J. Vis. Exp. (180), e63509, doi:10.3791/63509 (2022).

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