TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of <em>Drosophila</em> Embryos

Benjamin Bertin; Yoan Renaud; Rajaguru Aradhya; Krzysztof Jagla; Guillaume Junion

doi:10.3791/52985

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TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

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TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

17,491 Views

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10:26 min

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September 10, 2015

DOI:

10.3791/52985-v

DOI:

10.3791/52985-v

•

10:26 min

September 10, 2015

•

3 Views

Benjamin Bertin¹, Yoan Renaud¹, Rajaguru Aradhya², Krzysztof Jagla¹, Guillaume Junion¹

¹University of Clermont-Ferrand, ²Memorial Sloan Kettering Cancer Center

Summary

Translating Ribosome Affinity Purification (TRAP) is able to capture cell-type-specific translation of mRNA. Here we report the first TRAP protocol dedicated to isolation of mRNA in rare cell populations of Drosophila embryos.

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TRAP Translating Ribosome Affinity Purification Rare Cell Populations Drosophila Embryos MRNA Translation Cell-type-specific Translatome Analysis Polysomes Tagged Ribosomal Subunit Developmental Processes Disease Progression Cell-specific Gene Expression Microarray RNA-seq Gal4/UAS System Muscle Cells

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TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

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