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TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

JoVE 杂志
生物学

This content is Free Access.

JoVE 杂志生物学

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

17,464 Views

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10:26 min

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September 10, 2015

DOI:

10.3791/52985-v

DOI:

10.3791/52985-v

•

10:26 min

September 10, 2015

•

3 Views

Benjamin Bertin, Yoan Renaud, Rajaguru Aradhya, Krzysztof Jagla, Guillaume Junion

¹University of Clermont-Ferrand, ²Memorial Sloan Kettering Cancer Center

Tags

TRAP Translating Ribosome Affinity Purification Rare Cell Populations Drosophila Embryos MRNA Translation Cell-type-specific Translatome Analysis Polysomes Tagged Ribosomal Subunit Developmental Processes Disease Progression Cell-specific Gene Expression Microarray RNA-seq Gal4/UAS System Muscle Cells

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