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TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos
JoVE Journal
Biologia
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JoVE Journal Biologia
TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos

17,491 Views

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10:26 min

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September 10, 2015

DOI:

10.3791/52985-v

DOI:
10.3791/52985-v

•

10:26 min
September 10, 2015

•

3 Views
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1University of Clermont-Ferrand, 2Memorial Sloan Kettering Cancer Center

Summary

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Translating Ribosome Affinity Purification (TRAP) is able to capture cell-type-specific translation of mRNA. Here we report the first TRAP protocol dedicated to isolation of mRNA in rare cell populations of Drosophila embryos.

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TRAPTranslating Ribosome Affinity PurificationRare Cell PopulationsDrosophila EmbryosMRNA TranslationCell-type-specificTranslatome AnalysisPolysomesTagged Ribosomal SubunitDevelopmental ProcessesDisease ProgressionCell-specific Gene ExpressionMicroarrayRNA-seqGal4/UAS SystemMuscle Cells

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Bertin, B., Renaud, Y., Aradhya, R., Jagla, K., Junion, G. TRAP-rc, Translating Ribosome Affinity Purification from Rare Cell Populations of Drosophila Embryos. J. Vis. Exp. (103), e52985, doi:10.3791/52985 (2015).

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