Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Laser Capture Microdissection - A Demonstration…
JoVE Journal
Neuroscience

이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.

JoVE Journal Neuroscience
Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area
 

Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area

Article DOI: 10.3791/52336-v • 08:29 min • February 6th, 2015
February 6th, 2015 •
1, 1, 1
1Department of Basic Sciences, Mississippi State University College of Veterinary Medicine

챕터

요약
Automatic Translation

The isolation of individual dopamine neurons or the ventral tegmental area with direct or indirect immunohistochemistry is demonstrated using laser capture microdissection. Parameters for isolation of tissue from a glass slide using an infrared laser and from membrane slides using the combination of an infrared and ultraviolet laser are discussed.

Tags

Laser Capture Microdissection LCM Isolation Individual Cells Anatomical Regions Tissue Sections Microscope Slide Immunohistochemistry Dopamine Neurons Ventral Tegmental Area Tyrosine Hydroxylase IR Capture Laser UV Cutting Laser PEN Membrane Slide
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter