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Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area
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09:54 min
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August 10, 2012
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Dopamine is distinctly regulated in the midbrain nuclei, which contain the cell bodies and dendrites of the dopamine neurons. Here we describe a dissection and sample-handling approach to maximize results, and thus conclusions and insights, on dopamine regulation in the midbrain nuclei of the substantia nigra (SN) and ventral tegmental area (VTA) in rodents.
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Dopamine RegulationNeurotransmitterCNSLocomotor ActivityReward-related BehaviorMolecular DeficienciesNigrostriatal PathwayMesoaccumbens PathwayStriatumNucleus AccumbensTyrosine Hydroxylase ProteinTH PhosphorylationDopamine Transporter ProteinVesicular Monoamine Transporter 2 ProteinPost-translational ModificationsMidbrain
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Salvatore, M. F., Pruett, B. S., Dempsey, C., Fields, V. Comprehensive Profiling of Dopamine Regulation in Substantia Nigra and Ventral Tegmental Area. J. Vis. Exp. (66), e4171, doi:10.3791/4171 (2012).
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