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अलगाव और मानव का संवर्धन बढ़ी अस्थिजनन के लिए stromal कोशिकाओं वसा व्युत्पन्न
JoVE 신문
발생학
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JoVE 신문 발생학
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
DOI:

11:00 min

January 12, 2015

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Chapters

  • 00:05Title
  • 01:06Cell Isolation and Culture
  • 04:52Staining and Cell Sorting
  • 08:37Results: Isolation of Human Adipose-derived Stromal Cells with Increased Osteogenic Potential
  • 10:30Conclusion

Summary

자동 번역

The transcriptional heterogeneity within human adipose-derived stromal cells can be defined on the single cell level using cell surface markers and osteogenic genes. We describe a protocol utilizing flow cytometry for the isolation of cell subpopulations with increased osteogenic potential, which may be used to enhance craniofacial skeletal reconstruction.

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