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Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
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Developmental Biology
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JoVE Journal Developmental Biology
Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

DOI:
10.3791/52181-v

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11:00 min

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January 12, 2015

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, , , , , , , , , , , , ,
1Hagey Laboratory for Pediatric Regenerative Medicine, Department of Surgery, Division of Plastic and Reconstructive Surgery,Stanford University School of Medicine, 2Institute for Stem Cell Biology and Regenerative Medicine,Stanford University

Chapters

  • 00:05Title
  • 01:06Cell Isolation and Culture
  • 04:52Staining and Cell Sorting
  • 08:37Results: Isolation of Human Adipose-derived Stromal Cells with Increased Osteogenic Potential
  • 10:30Conclusion

Summary

Automatic Translation

Automatic Translation

The transcriptional heterogeneity within human adipose-derived stromal cells can be defined on the single cell level using cell surface markers and osteogenic genes. We describe a protocol utilizing flow cytometry for the isolation of cell subpopulations with increased osteogenic potential, which may be used to enhance craniofacial skeletal reconstruction.

Tags

Keywords: Adipose-derived Stromal CellsASCsBone Marrow-derived Mesenchymal Stromal CellsBM-MSCsOsteogenesisStromal Vascular FractionSVFCritical-sized Calvarial Defects

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